Enquiry E-CATALOGUE

Kao & Michayluk Basal Salt Mixture

  • Product ID: K413

Introduction:

Contains the macro- and micronutrients as described by Kao & Michayluk (1975).
This medium was originally developed for the culture of Vicia hajastana protoplasts. The culture of low cell densities (1-2 cells/mL) is enhanced when the medium is supplemented with organic acids, sugar alcohols, sugars, plant growth regulators and amino acids. (See K427) Cell growth and division could also be enhanced by raising the concentrations of Calcium Chloride from 1 to 5 mM.

K413 Kao & Michayluk Basal Salt Mixture



Properties

Form: Powder
Appearance: White to Yellow
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 3.65g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro and micronutrients as described by Kao & Michayluk (1975).

Formula (mg/L)
Ammonium Nitrate 600
Boric Acid 3
Calcium Chloride, Anhydrous  453
Cobalt Chloride·6H2O  0.025
Cupric Sulfate·5H2O  0.025
Na2 EDTA·2H2O  37.26
Ferrous Sulfate·7H2O  27.85
Magnesium Sulfate, Anhydrous 146.55
Manganese Sulfate·H2O 10
Molybdic Acid (Sodium Salt)·2H2O  0.25
Potassium Chloride 300
Potassium Iodide 0.75
Potassium Nitrate  1900
Potassium Phosphate, Monobasic  170
Zinc Sulfate·7H2O  2

Application Notes
Plant species: Vicia hajastana (Kao & Michayluk, 1975); Vicia faba (Binding & Nehls, 1978); Daucus carota (Grzebelus et al, 2012).


This medium was originally developed for the culture of Vicia hajastana protoplasts. The culture of low cell densities (1-2 cells/ml) is enhanced when the medium is supplemented with organic acids, sugar alcohols, sugars, plant growth regulators and amino acids. (See K427) Cell growth and division could also be enhanced by raising the concentrations of calcium chloride from 1 to 5 mM.


References
Binding, H. and Nehls, R. (1978) Regeneration of Isolated Protoplasts of Vicia faba L. Z. Pflanzenpbysiol. 88, 327-332. Grzebelus E, M Szklarczyk & R Baranski (2012)
An improved protocol for plant regeneration from leafand hypocotyl-derived protoplasts of carrot. Plant Cell Tiss Organ Cult 109:101-109. Kao, KN and MR Michayluk (1975)
Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105-110.

Life Technologies (India) Pvt Ltd. 

306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India)
Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444,
Mobile# +91-98105-21400, Toll Free# 1800-120-2434
Email# customerservice@lifetechindia.com