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Hosta Multiplication Medium

  • Product ID: H436

Introduction:

Stage II medium. Modified M & S with (mg/L): 0.1 6-BA, 160 Adenine Hemisulfate, 500 Casein Hydrolysate, & 0.5 NAA.
Contains the macro- and micronutrients as described by Murashige & Skoog (1962) and the vitamins described by Linsmaier & Skoog (1965). Also contains sucrose andagar.


H436 Hosta Multiplication Medium Stage II Medium




Properties

Form: Powder
Appearance: White to Yellow
Application: Plant Tissue Culture
Solubility: Partially Soluble in Cold Water. Soluble in Hot Water
Typical Working Concentration: 43.39 g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965). Also contains agar and sucrose. Plant Tissue Culture Tested
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Chloride, Anhydrous 332.2
Cobalt Chloride•6H2O 0.025
Cupric Sulfate•5H2O 0.025
Disodium EDTA •2H2O 37.26
Ferrous Sulfate•7H2O 27.8
 Magnesium Sulfate, Anhydrous  180.7
 Manganese Sulfate.H2O 16.9
 Molybdic Acid (Sodium Salt). 2H2O  0.25
 Potassium Iodide 0.83
 Potassium Nitrate  1900
 Potassium Phosphate, Monobasic  300
 S odium Phosphate, Monobasic  170
 Zinc Sulfate.7H2O 8.6
 Adenine Hemisulfate 160
Agar  8000
 6-Benzylaminopurine (BA) 0.1
 Casein, Enzymatic Hydrolysate 500
 Glycine (Free Base) 2
 myo-Inositol 100
 a-Naphthaleneacetic Acid  0.5
 Sucrose  30,000
 Thiamine.HCl 0.4

Application Notes
Plant species: Hosta

References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497.
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100-127.

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