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Introduction:
Stage II medium. Modified M & S with (mg/L): 0.1 6-BA, 160 Adenine Hemisulfate, 500 Casein Hydrolysate, & 0.5 NAA.
Contains the macro- and micronutrients as described by Murashige & Skoog (1962) and the vitamins described by Linsmaier & Skoog (1965). Also contains sucrose andagar.
H436 Hosta Multiplication Medium Stage II Medium
| Form: | Powder |
| Appearance: | White to Yellow |
| Application: | Plant Tissue Culture |
| Solubility: | Partially Soluble in Cold Water. Soluble in Hot Water |
| Typical Working Concentration: | 43.39 g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients as described by Murashige and Skoog (1962) and the vitamins described by Linsmaier and Skoog (1965). Also contains agar and sucrose. Plant Tissue Culture Tested |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 1650 |
| Boric Acid | 6.2 |
| Calcium Chloride, Anhydrous | 332.2 |
| Cobalt Chloride•6H2O | 0.025 |
| Cupric Sulfate•5H2O | 0.025 |
| Disodium EDTA •2H2O | 37.26 |
| Ferrous Sulfate•7H2O | 27.8 |
| Magnesium Sulfate, Anhydrous | 180.7 |
| Manganese Sulfate.H2O | 16.9 |
| Molybdic Acid (Sodium Salt). 2H2O | 0.25 |
| Potassium Iodide | 0.83 |
| Potassium Nitrate | 1900 |
| Potassium Phosphate, Monobasic | 300 |
| S odium Phosphate, Monobasic | 170 |
| Zinc Sulfate.7H2O | 8.6 |
| Adenine Hemisulfate | 160 |
| Agar | 8000 |
| 6-Benzylaminopurine (BA) | 0.1 |
| Casein, Enzymatic Hydrolysate | 500 |
| Glycine (Free Base) | 2 |
| myo-Inositol | 100 |
| a-Naphthaleneacetic Acid | 0.5 |
| Sucrose | 30,000 |
| Thiamine.HCl | 0.4 |
Application Notes
Plant species: Hosta
References
Murashige, T and F Skoog. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiologia Plantarum. 15: 473-497.
Linsmaier, EM and F Skoog. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 18: 100-127.