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Gamborg B-5 Basal Medium

  • Product ID: G398

Introduction:

Contains the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968).
This medium was developed for the initiation and growth of soybean cell suspensions. This medium contains no Ammonium Nitrate; it does contain Ammonium Sulfate and increased levels of Potassium Nitrate. Concentrations of NH4+ over 2 mM inhibited cell growth.


G398 Gamborg B-5 Basal Medium




Properties

Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 3.21g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Gamborg, et al (1968). pH = 3.5 – 4.5

Formula (mg/L)
Ammonium Sulfate  134
Boric Acid 3
Calcium Chloride, Anhydrous  113.24
Cobalt Chloride·6H2O  0.025
Cupric Sulfate·5H2O  0.025
Na2 EDTA·2H2O  37.26
Ferrous Sulfate·7H2O  27.8
Magnesium Sulfate, Anhydrous  122.09
Manganese Sulfate·H2O  10
Molybdic Acid (Sodium Salt)·2H2O  0.25
Potassium Iodide  0.75
Potassium Nitrate  2500
Sodium Phosphate Monobasic  150
Zinc Sulfate·7H2O 2
myo-Inositol  100
Nicotinic Acid (Free Acid)  1
Pyridoxine·HCl  1
Thiamine·HCl  10

Application Notes
Plant Species: Soybean (Glycine max) This medium was developed for the initiation and growth of soybean cell suspensions.
This medium contains no ammonium nitrate; it does contain ammonium sulfate and increased levels of potassium nitrate.Concentrations of NH4 + over 2 mM inhibited cell growth. Thiamine, an essential vitamin, is at a concentration 10x that of Linsmaier & Skoog Medium.


References
Gamborg, OL, RA Miller, K Ojima. 1968. Nutrient Requirements of suspension cultures of soybean root cells. Exp. Cell Research 50: 151-158.

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