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Introduction:
Contains the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968).
This medium was developed for the initiation and growth of soybean cell suspensions. This medium contains no Ammonium Nitrate; it does contain Ammonium Sulfate and increased levels of Potassium Nitrate. Concentrations of NH4+ over 2 mM inhibited cell growth.
G398 Gamborg B-5 Basal Medium
Properties
| Properties | |
|---|---|
| Form: | Powder |
| Appearance: | White to Yellow Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 3.21g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients and vitamins as described by Gamborg, et al (1968). pH = 3.5 – 4.5 |
| Formula (mg/L) | |
|---|---|
| Ammonium Sulfate | 134 |
| Boric Acid | 3 |
| Calcium Chloride, Anhydrous | 113.24 |
| Cobalt Chloride·6H2O | 0.025 |
| Cupric Sulfate·5H2O | 0.025 |
| Na2 EDTA·2H2O | 37.26 |
| Ferrous Sulfate·7H2O | 27.8 |
| Magnesium Sulfate, Anhydrous | 122.09 |
| Manganese Sulfate·H2O | 10 |
| Molybdic Acid (Sodium Salt)·2H2O | 0.25 |
| Potassium Iodide | 0.75 |
| Potassium Nitrate | 2500 |
| Sodium Phosphate Monobasic | 150 |
| Zinc Sulfate·7H2O | 2 |
| myo-Inositol | 100 |
| Nicotinic Acid (Free Acid) | 1 |
| Pyridoxine·HCl | 1 |
| Thiamine·HCl | 10 |
Application Notes
Plant Species: Soybean (Glycine max) This medium was developed for the initiation and growth of soybean cell suspensions.
This medium contains no ammonium nitrate; it does contain ammonium sulfate and increased levels of potassium nitrate.Concentrations of NH4 + over 2 mM inhibited cell growth. Thiamine, an essential vitamin, is at a concentration 10x that of Linsmaier & Skoog Medium.
References
Gamborg, OL, RA Miller, K Ojima. 1968. Nutrient Requirements of suspension cultures of soybean root cells. Exp. Cell Research 50: 151-158.