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Introduction:
Contains the macro- and micronutrients as described by Gresshoff & Doy (1974).
This medium was originally developed for the growth of haploid callus and plants from anthers of Arabidopsis thalian a. Anthers cultured in late prophase of meiosis gave best results for haploid plantlet development. This medium has been used for haploid plantlet production in tomato, barley, and grape.
G371 Gresshoff & Doy Basal Medium
| Synonyms: | GA3; Gibberellin A3 |
| CAS: | 77-06-5 |
| Formula: | C19H22O6 |
| Mol. Weight: | 346.41 |
Properties
| Form: | Powder |
| Appearance: | White to Yellow Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Soluble in Hot Water |
| Typical Working Concentration: | 2.71g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients and vitamins as described by Gresshoff and Doy (1974). |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 1000 |
| Boric Acid | 0.3 |
| Calcium Nitrate | 241.2 |
| Cobalt Chloride·6H2O | 0.025 |
| Cupric Sulfate·5H2O | 0.025 |
| Na2 EDTA·2H2O | 37.25 |
| Ferrous Sulfate·7H2O | 27.85 |
| Magnesium Sulfate, Anhydrous | 17.1 |
| Manganese Sulfate·H2O | 1 |
| Molybdic Acid (Sodium Salt)·2H2O | 0.025 |
| Potassium Chloride | 65 |
| Potassium Iodide | 0.8 |
| Potassium Nitrate | 1000 |
| Potassium Phosphate, Monobasic | 300 |
| Zinc Sulfate·7H2O | 0.3 |
| D-Biotin | 0.2 |
| Glycine (Free Base) | 4 |
| myo-Inositol | 10 |
| Nicotinic Acid (Free Acid) | 0.1 |
| Pyridoxine·HCl | 0.1 |
| Thiamine·HCl | 1 |
Application Notes
Plant Species: Grapevine (Vitis), Arabidopsis, tomato, barley (Gresshoff & Doy, 1974); Trifolium repens (Gresshoff, 1980).
This medium was originally developed for the growth of haploid callus and plants from anthers of Vitis vinifera. Anthers cultured in late prophase of meiosis gave best results for haploid plantlet development
References
Gresshoff, PM and CH Doy. (1974) Derivation of a haploid cell line from Vitis vinifera and the importance of the stage of meiotic development of anthers for haploid culture of this and other genera. Z. Pflanzenphysiol. 73: 132-141.
Gresshoff, PM (1980) In vitro culture of white clover: callus, suspension protoplast culture and plant regeneration . Bot. Gaz. (Chicago), 141: 157-164