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Life Technologies (India) Pvt Ltd. 306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India) Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444, Mobile# +91-98105-21400, Toll Free# 1800-120-2434 Email# customerservice@lifetechindia.com |
Introduction:
Description:
Purified Agar (A175: 8 g)
GelzanT (G3251: 2 g)
AgargellanT (A133: 5 g)
Carrageenan (C2000: 10 g )
| Solubility | Variable |
| Physical Form | Solid |
| Storage Temp. | Room Temperature |
| Other Notes | Plant Tissue Culture Tested |
| UPC / SKU | G3500 |
| CAS NUMBER | Variable |
| Gelling Strength | Variable |
| Storage Temp. | Room Temperature |
| Tariff Code | 1302.31.0000 |
| Risk Info (R) | 36/37/38 |
| Safety Info (S) | 36 |
| No information available |
G3500 Gelling Agent Sample Pack
Sample Pack Contents:
• A111- Tissue Culture Grade Agar: 9g
• A175- Purified Agar: 8g
• A133- Agargellan™: 5g
• C2000- Carrageenan: 10 g
• G3251- Gelzan™: 2g
Details:
A111- Tissue Culture Grade Agar: The most common gelling agent used in plant tissue culture. Gel Strength: ≥900 (typically 900-1100) g/cm2 . It is used at a wide range of concentrations from 6 g/L for a slightly firm gel to 10 g/L for a brick-like gel; however, 6-8 g/L is the more commonly used range. It is derived from red seaweeds in the genus Gracilaria.
A175- Purified Agar: The most common gelling agent used in plant tissue culture. Gel Strength: 700-1100 g/cm2 . It is used at a wide range of concentrations from 6 g/L for a slightly firm gel to 10 g/L for a brick-like gel; however, 6-8 g/L is the more commonly used range. It is derived from red seaweeds in the genus Gelidium.
A133- Agargellan™: A proprietary blend of agar and gellan gum. Not recommended for use with media with high calcium concentrations, such as DKW or Quoirin & Lepoivre Medium, as gelling ability will be affected. It is typically used at a concentration of 3.5 – 5.0 g/L for tissue culture.
C2000- Carrageenan: Typically used at a wide range of concentrations from 6 to 10 g/L. Should be the last component added as the medium will become viscous, since carrageenan is a water-soluble polymer. Carrageenan should also be added slowly to an agitated medium to help prevent clumping. A lumpy suspension of carrageenan will not dissolve uniformly when autoclaved.
G3251- Gelzan™: A gellan gum that is sometimes referred to as “Gelrite™” by some suppliers. However, Gelrite™ is no longer produced, and this product has a lower gel strength than the original Gelrite™. Gellan gum is produced by bacterial fermentation from a pure culture of Sphingomonas elodea. It is typically used at a concentration of 2-4 g/L for tissue culture. Gelzan™ is a trademarked product of CP Kelco U.S., Inc. Not recommended for use with high-calcium media such as DKW or Quoirin & LePoivre