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Introduction:
Gentamicin sulfate is an aminoglycoside antibiotic that is effective against many gram-negative bacteria (e.g., Brucella, Escherichia, Enterobacter, Francisella, Yersinia, etc.) and some strains of staphylococci.
G3410 Gentamicin Sulfate Solution 100 mg/mL
| Synonyms: | Gentamycin Sulfate |
| CAS: | 1405-41-0 |
| Formula: | C21H43N5O7 • H2SO4 |
| Mol. Weight: | 575.67 |
Properties
| Form: | Liquid |
| Appearance: | Clear, Colorless Liquid |
| Application: | Plant Tissue Culture Antibiotic |
| Solubility: | Miscible in Water |
| Storage Temp: | 2-8 °C |
| Typical Working Concentration: | Varies |
| Storage Temp of Stock Solution: | 2-8 °C |
| Other Notes: | Plant Tissue Culture Tested |
Application Notes
Gentamicin Sulfate is an aminoglycoside antibiotic that is effective against many Gram-negative bacteria, e.g., Brucella, Escherichia, Enterobacter, Francisella, Yersinia, etc., and some strains of staphylococci. It inhibits the initiation, elongation and termination of protein synthesis by binding to the 30S subunit and sometimes the 50S subunit of the bacterial ribosome.2 Gentamicin is suitable for plant tissue culture use as it is stable for autoclaving; however, it has been reported that gentamicin inhibits growth of tobacco, sapiglossis, romaine lettuce and artichoke at high concentration ranging from 50 to 100 µg/mL.3, 4
Minimum inhibitory concentration (MIC) of gentamicin has been reported for many bacteria. MIC for Escherichia coli is 0.05 µg/mL, Bacteroides spp. is greater than 128 µg/mL, and Clostridium spp. is 112 µg/mL. 5
PhytoTechnology Laboratories® also carries gentamicin sulfate solution 50 mg/mL, Product No. G3350, and gentamicin sulfate powder, Product No. G570.
Please note: It is the sole responsibility of the purchaser to determine the appropriateness of this product for the specific plants that are being cultured and applications that are being used.
References
1. Merck 13, 4403
2. Martindale: The Complete Drug Reference, 35th ed., Paul S. Blake, Ed. (Royal Pharmaceutical Society, 2007), p. 252.
3. David A. Eichholtz, Paul M. Hasegawa and Henry A. Robitaille. 1982. Effects of gentamicin on growth of shoot initiation from cultured tobacco callus and Salpiglossis leaf discs. In Vitro Cellular & Developmental Biology – Plant. 18(1):12-14, DOI: 10.1007/BF02796380.
4. J. H. Dodds and L. W. Roberts. 1981. Some inhibitory effects of gentamicin on plant tissue cultures. In Vitro Cellular & Developmental Biology – Plant. 17(6):467-470, DOI: 10.1007/BF02633507.
5.Olsen, P. and L. Dragsted. “918. Gentamicin (Who Food Additives Series 41)”.IPCS Inchem. http://www.inchem.org/documents/jecfa/jecmono/v041je05.htm (accessed 5 Mar 2013).