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De Greef & Jacobs Basal Medium

  • Product ID: D2206

Introduction:

Contains the macro- and micronutrients and vitamins as described by De Greef and Jacobs (1979).

D2206 DE GREEF & JACOBS BASAL MEDIUM


Properties


Form: Powder
Appearance: White to Light Yellow
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 3.91g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by De Greef & Jacobs (1979). Plant Tissue Culture Tested Unadjusted pH (approximately): 4.0 – 5.0
Formula (mg/L)
Ammonium Sulfate 400.0
 Potassium Iodide 1.58
 Boric Acid 10.62
 Potassium Nitrate 2,000
 Calcium Chloride, Anhydrous  226.5
 Sodium Phosphate, Monobasic 250.0
Cobalt Chloride 0.0025
Sodium Molybdate 0.0025
 Cupric Sulfate  0.0025
 Zinc Sulfate 7H2O 1.06
 Ferrous Sulfate 7H2O 27.8
 myo-Inositol  100.0
 Magnesium Sulfate Anhydrous 244.33
 Nicotinic Acid  1.0
 Manganese Sulfate H2O 1.68
Pyridoxine HCl 1.0
 Na2 EDTA 2H2O 37.26
 Thiamine HCl 10.0
 Potassium Chloride 600.0

Application Notes
Originally used in the tissue culture of sugar beets.


References
De Greef W, Jacobs M. 1979. In vitro culture of sugar beet: description of a cell line with high regeneration capacity. Plant Science Letters 17, 55-61.

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