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Introduction:
D189 DKW Basal Medium
Synonym: DKW Basal Salts w/ Sucrose;
Driver and Kuniyuki Walnut medium
| Form: | Powder |
| Appearance: | White to Yellow |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 35.22 g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients as described by Driver and Kuniyuki (1984) and McGranahan, et al. (1987). Also contains sucrose. pH = 3.5 – 4.5 |
| Formula (mg/L) | |
|---|---|
| Ammonium Nitrate | 1416 |
| Boric Acid | 4.8 |
| Calcium Chloride, Anhydrous | 112.5 |
| Calcium Nitrate | 1367 |
| Cupric Sulfate·5H2O | 0.25 |
| Na2 EDTA·2H2O | 45.4 |
| Ferrous Sulfate·7H2O | 33.8 |
| Magnesium Sulfate, Anhydrous | 361.49 |
| Manganese Sulfate·H2O | 33.5 |
| Molybdic Acid (Sodium Salt)·2H2O | 0.39 |
| Nickel Sulfate·6H2O | 0.005 |
| Potassium Phosphate, Monobasic | 265 |
| Potassium Sulfate | 1559 |
| Zinc Nitrate·6H2O | 17 |
| Sucrose | 30,000 |
Application Notes
Plant species: Northern California Walnut (Juglans hindsii). This medium was developed for the multiplication of shoots from nodal explants. The medium was supplemented with 4.5 µM BA and 5 nM IBA. Rooting the shoots was enhanced by dipping the basal ends of the shoots in 5 mM IBA prior to transferring to the greenhouse
References
Driver, J.A. and A.H. Kuniyuki. 1984. In vitro propagation of Paradox walnut rootstock. HortScience 19:507–509. McGranahan, GH, et al. 1987. In: Bonga, JB and DJ Durzan, Editors, Cell and Tissue Culture in Forestry. Martinus Nijhoff, Dordrecht, pp 261-271.