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DKW Basal Medium with 30 g/L Sucrose

  • Product ID: D189

Introduction:


D189 DKW Basal Medium


Synonym: DKW Basal Salts w/ Sucrose;
Driver and Kuniyuki Walnut medium


Properties


Form: Powder
Appearance: White to Yellow
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 35.22 g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients as described by Driver and Kuniyuki (1984) and McGranahan, et al. (1987). Also contains sucrose. pH = 3.5 – 4.5
Formula (mg/L)
Ammonium Nitrate 1416
Boric Acid 4.8
 Calcium Chloride, Anhydrous 112.5
 Calcium Nitrate 1367
Cupric Sulfate·5H2O 0.25
Na2 EDTA·2H2O 45.4
 Ferrous Sulfate·7H2O 33.8
Magnesium Sulfate, Anhydrous 361.49
Manganese Sulfate·H2O 33.5
 Molybdic Acid (Sodium Salt)·2H2O  0.39
 Nickel Sulfate·6H2O 0.005
 Potassium Phosphate, Monobasic  265
 Potassium Sulfate 1559
 Zinc Nitrate·6H2O  17
 Sucrose 30,000

Application Notes
Plant species: Northern California Walnut (Juglans hindsii). This medium was developed for the multiplication of shoots from nodal explants. The medium was supplemented with 4.5 µM BA and 5 nM IBA. Rooting the shoots was enhanced by dipping the basal ends of the shoots in 5 mM IBA prior to transferring to the greenhouse


References
Driver, J.A. and A.H. Kuniyuki. 1984. In vitro propagation of Paradox walnut rootstock. HortScience 19:507–509. McGranahan, GH, et al. 1987. In: Bonga, JB and DJ Durzan, Editors, Cell and Tissue Culture in Forestry. Martinus Nijhoff, Dordrecht, pp 261-271.

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