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DCR Basal Salt Mixture

  • Product ID: D146

Introduction:

Contains macro- and micronutrients as described by Gupta & Durzan (1985).
This medium was developed to promote shoot proliferation. This formulation has approximately ¼ the concentration of NH4NO3 and KNO3 compared to MS. Additional Ca+2 and NO3- ions are supplied by Ca(NO3)2.

D146 DCR Basal Salt Mixture


Properties

Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 1.64g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Chu et al. (1975). pH = 3.5 – 4.5
Formula (mg/L)
Ammonium Nitrate 400
Boric Acid 6.2
Calcium Chloride, Anhydrous 64.14
Calcium Nitrate 386.31
Cobalt Chloride·6H2O 0.025
Cupric Sulfate·5H2O 0.25
Na2 EDTA·2H2O 37.3
Ferrous Sulfate·7H2O 27.8
Magnesium Sulfate, Anhydrous 180.7
Manganese Sulfate·H2O 22.3
Molybdic Acid (Sodium Salt)·2H2O  0.25
Nickel Chloride·6H2O 0.025
Potassium Iodide 0.83
Potassium Nitrate 340
Potassium Phosphate, Monobasic  170
Zinc Sulfate·7H2O 8.6

Application Notes
Plant Tissue Culture Tested
Plant Species: Douglas Fir (Pseudotsuga spp.) and Sugar Pine (Pinus lambertiana)
This medium was developed to promote shoot proliferation. This formulation has approximately ¼ the concentration of NH4NO3 and KNO3 compared to MS. Additional Ca+2 and NO3 - ions are supplied by Ca(NO3)2.


References
Gupta, PK and KJ Durzan. 1985. Shoot multiplication from mature Douglas-fir and sugar pine. Plant Cell Reports 4:177-179.

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