Enquiry E-CATALOGUE

Chu's N6 Basal Salt Mixture

  • Product ID: C416

Introduction:

Contains the macro- and micronutrients as described by Chu (1975).
Chu (N6) Medium was developed to promote the initiation, growth, and differentiation of callus from rice pollen cultures.

C416 Chu N6 Basal Salt Mixture

Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 3.98g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Chu et al. (1975). pH = 3.5 – 4.5
Formula (mg/L)
Ammonium Sulfate  463
Boric Acid  1.6
Calcium Chloride, Anhydrous  125.33
Na2 EDTA·2H2O  37.25
Ferrous Sulfate·7H2O 27.85
Magnesium Sulfate, Anhydrous  90.37
Manganese Sulfate·H2O 3.3
Potassium Iodide 0.8
Potassium Nitrate  2830
Potassium Phosphate, Monobasic  400
Zinc Sulfate·7H2O 1.5

Application Notes
Plant Tissue Culture Tested
Plant species: rice (Oryza sativa ) Chu (N6) Medium was developed to promote the initiation, growth, and differentiation of callus from rice pollen cultures.
Ammonium nitrate has been replaced by ammonium sulfate. The molar concentration of NH4 + is 7.0 mM compared to 20.6mM for MS.


References
Chu CC, CC Wang, CS Sun, C Hsu, KC Yin, CY Chu and FY Bi. (1975) Scientia Sinic. 18: 659- 668.

Life Technologies (India) Pvt Ltd. 

306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India)
Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444,
Mobile# +91-98105-21400, Toll Free# 1800-120-2434
Email# customerservice@lifetechindia.com