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Introduction:
Activated charcoal absorbs and acts as a reservoir for growth inhibitory secondary products given off by plant tissue. Acid washed.
Synonyms: Carbon Black; Activated Carbon.
C325 Charcoal, Activated, Acid Washed
Properties
| Form: | Powder |
| Appearance: | Black Fine Powder |
| Application: | Orchid Media Supplement |
| Solubility: | Insoluble - Forms Suspension with Water |
| Typical Working Concentration: | 1 to 5 grams per liter |
| Storage Temp: | Room Temperature |
| Other Notes: | Plant Tissue Culture Tested |
Application Notes
Activated charcoal
(AC)
works by ad
sorbing toxic substances (e.g., phenols
, inorganic compounds
, and other
organic
impurities
) secreted by plant tissues or present in media ingredient that would otherwise affect growth.
Due
to its ability to a
dsorb many impurities, usages of AC
in culture media had been reported
to improve and/or
stimulate growth, organogenesis and embryogenesis for
many
plant species.2.3
Typical concentration of AC
ranges from 1 to 5 g/L
which
has been reported to help reduce polyphenol exudation in
immature embryos of
Sorghum bicolour
; however,
a low concentration of
0.5 g/L has been reported to help reduce
polyphenol from
Aristolochia indica
.3
For orchid tissue culture, AC
is also
com
monly used in orchid media to adsorb phenolics compounds secreted by
wounded plant tissues
to prev
ent
darkening of media
which would
inhibit
plant growth.
Seedlings
of
Phalaenopsis
when
cultured on
media supplemented with AC
at 0.5 g/L
ha
ve shown significant
shoot and root development;
while at 3 g/L of AC help
ed
increase root development of
Cymbidium
and
Dendrobium
.4
While m
any a
dvantages has been reported for the use
of
activated
charcoal
, a report of AC interference with
growth hormones
has been report in which it a
dsorbed the required growth hormones
for the growth development
of
callus growth of
Nicotiana tabacum
cv. Wisconsin
38.5
Please Note: It is the sole responsibility of the purchaser to determine the appropriateness of this product for the
specific plants that are being cultured and applications that are being used
References
1. Merck 13, 1818
2. George G. 1993. Plant Pr
opagation by Tissue Culture, Part 1: The Technology. England: Exegetics
Limited, 574 pp.
3. Thomas, T. Dennis. 2008. The Role of Activated Charcoal in Plant Tissue Culture. Biotechnology Advances. 26:618-631