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Chee & Pool C2D Vitis Medium

  • Product ID: C287

Introduction:

Contains the macro- and micronutrients as described by Chee & Pool (1987).
This medium was developed to improve grape shoot multiplication. This formulation has reduced levels of Chloride, Iodide, and manganese compared to MS. Calcium Chloride was replaced by Calcium Nitrate, thus improving the quality of grape shoots.

C287 Chee & Pool C2d Vitis Basal Medium

Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water Soluble
Typical Working Concentration: 4.49g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients and vitamins as described by Chee and Pool (1987). pH = 3.5 – 4.5
Formula (mg/L)
Ammonium Nitrate 1650
Boric Acid 6.2
Calcium Nitrate 492.3
Cobalt Chloride·6H2O 0.025
Cupric Sulfate·5H2O 0.025
Na2 EDTA·2H2O 37.3
Ferrous Sulfate·7H2O 27.8
Magnesium Sulfate, Anhydrous 180.6
Manganese Sulfate·H2O 0.845
Molybdic Acid (Sodium Salt)·2H2O 0.25
Potassium Nitrate 1900
Potassium Phosphate, Monobasic 170
Zinc Sulfate·7H2O 8.6
myo-Inositol 10
Nicotinic Acid (Free Acid) 1.0
Pyridoxine·HCl 1.0
Thiamine·HCl 1.0

Application Notes
Plant Tissue Culture Tested
Plant Species: Grape (Vitis spp) This medium was developed to improve grape shoot multiplication. This formulation has reduced levels of chloride, Iodide, and manganese compared to MS. Calcium chloride was replaced by calcium nitrate, thus improving the quality of grape shoots.


References
Chee, R and RM Pool. 1987. Improved inorganic media constituents for in vitro shoot multiplication of Vitis. Sci. Hort. 32: 85.

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