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Carrot Shoot Development Basal Medium

  • Product ID: C222

Introduction:

Contains the macro- and micronutrients, vitamins, and plant growth regulators required to initiate shoots from carrot callus.

C222 Carrot Shoot Development Basal Medium


Properties
Form: Powder
Appearance: White to Yellow Powder
Application: Plant Tissue Culture
Solubility: Water
Typical Working Concentration: 3.21g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients, vitamins, and plant growth regulators required to initiate carrot callus from root tissue. Requires the addition of sucrose (e.g., 30 g/L) and a gelling agent (e.g., 6-8 g/L agar) pH = 3.5 – 4.5; should be adjusted to pH 5.6 – 5.8
Formula (mg/L)
Ammonium Sulfate 134
Boric Acid 3
Calcium Chloride, Anhydrous 113.24
Cobalt Chloride·6H2O 0.025
Cupric Sulfate·5H2O 0.025
Na2 EDTA·2H2O 37.3
Ferrous Sulfate·7H2O 27.8
Magnesium Sulfate, Anhydrous 122.09
Manganese Sulfate·H2O 10
Molybdic Acid (Sodium Salt)·2H2O 0.25
Potassium Iodide 0.75
Potassium Nitrate 2500
Sodium Phosphate Monobasic 150
Zinc Sulfate·7H2O 2
2,4-Dichlorophenoxyacetic Acid 1
myo-Inositol 100
Nicotinic Acid (Free Acid) 1
Pyridoxine·HCl 1
Thiamine·HCl 10

Application Notes
Plant Tissue Culture Tested
Plant species: Carrot (Daucus carota)

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