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Introduction:
Contains the macro- and micronutrients, vitamins, and plant growth regulators required to initiate shoots from carrot callus.
C222 Carrot Shoot Development Basal Medium
| Properties | |
|---|---|
| Form: | Powder |
| Appearance: | White to Yellow Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 3.21g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients, vitamins, and plant growth regulators required to initiate carrot callus from root tissue. Requires the addition of sucrose (e.g., 30 g/L) and a gelling agent (e.g., 6-8 g/L agar) pH = 3.5 – 4.5; should be adjusted to pH 5.6 – 5.8 |
| Formula (mg/L) | |
|---|---|
| Ammonium Sulfate | 134 |
| Boric Acid | 3 |
| Calcium Chloride, Anhydrous | 113.24 |
| Cobalt Chloride·6H2O | 0.025 |
| Cupric Sulfate·5H2O | 0.025 |
| Na2 EDTA·2H2O | 37.3 |
| Ferrous Sulfate·7H2O | 27.8 |
| Magnesium Sulfate, Anhydrous | 122.09 |
| Manganese Sulfate·H2O | 10 |
| Molybdic Acid (Sodium Salt)·2H2O | 0.25 |
| Potassium Iodide | 0.75 |
| Potassium Nitrate | 2500 |
| Sodium Phosphate Monobasic | 150 |
| Zinc Sulfate·7H2O | 2 |
| 2,4-Dichlorophenoxyacetic Acid | 1 |
| myo-Inositol | 100 |
| Nicotinic Acid (Free Acid) | 1 |
| Pyridoxine·HCl | 1 |
| Thiamine·HCl | 10 |
Application Notes
Plant Tissue Culture Tested
Plant species: Carrot (Daucus carota)