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Coconut Water

  • Product ID: C195

Introduction:

Coconut water is a natural, no additive supplement of mainly vitamins and amino acids for plant tissue culture.

C195 Coconut Water



Properties

Form: Liquid
Appearance: Clear, Colorless to Slight Yellow Tint
Application: Plant Media Supplement
Solubility: Miscible with Water
Typical Working Concentration: 25 to 50 mL/L
Storage Temp: 2 to 8 °C
Other Notes: Plant Tissue Culture Tested
Sterile Filtere

Application Notes
Coconut water has been shown to stimulate shoot proliferation in man y plant species . This is most likely due to its unique composition of nutrients and natural phytohormones (e.g. zeatin and kinetin) (Mamaril et al ., 1988). It is most commonly used in orchid tissue culture as a medium supplement. Introduced by Overbeek et al. in 1940, coconut water was used as a new component of nutrient medium for callus cultures (1941) and has since become a popular additive in tissue culture media.

PhytoTechnology Laboratories® also carries Coconut Powder, Product No. C187, and Freeze Dried Organic Coconut Powder, Product No. C1780.

References

Afroz A, Z. Chaudhry, U. Rashid, M.R. Khan and G.M. Ali (2010) Enhanced regeneration in explants of tomato (Lycopersicon esculentum L.) with the treatment of coconut water. African Journal of Biotechnology 9(24), pp. 3634- 3644.
Alam MF, Sinha P, Hakim ML.(2010) Micropropagation of Vanda teres (Roxb.) Lindle. Methods Mol Biol . 589:21- 8. Al-Khayri, JM (2010) Somatic embryogenesis of date palm ( Phoenix dactylifera L.) improved by coconut water. Biotechnology , 9: 477- 484.
DOI: 10.3923/biotech.2010.477.484 Baskaran, P and N. Jayabalan (2005) An Efficient Micropropagation System for Eclipta alba: A Valuable Medicinal Herb. In Vitro Cellular & Developmental Biology . Plant, 41:4 Pp. 532- 539.
Jayasih, Y. and Wattimena, D.I.G. (1994) The effect of coconut water and zeolite on micropropagation of potato shoot. Acta Hort. (ISHS) 369:451 -454 http://www.actahort.org/books/369/369_49.htm
Kunisaki J, Araki A, Sagawa Y (2003) Micropropagation of 'awa (kava, Piper methysticum ). Honolulu (HI): University of Hawaii. 11 p. Biotechnology ; BIO -4. http://hdl.handle.net/10125/2842
Mandal AB, A. Maiti and Elanchezhian R. (2002) In vitro micropropagation of Ananas comosus L. (Merr.) var . Queen. Jour. Appl. Hort . 4(2):107- 112.
Martin KP (2007) Micropropagation of the bamboo orchid ( Arundina graminifolia (d. Don) hochr.) through protocorm -like -bodies using node explants. Propagation of Ornamental Plants 7(2): 97- 100.
Modi, P.; Banerjee, M. (2009) Effect of growth regulator and additives (AdSO4, zeatin and coconut water) on micropropagation of Rauvolfia serpentina . Journal of Applied Bioscience , v. 35(1): p. 79- 81.
Nasib A, K. Ali and S. Khan (2008) An optimized and improved method for the i n vitro propagation of kiwifruit ( Actinidia deliciosa ) using coconut water. Pak. J. Bot ., 40(6): 2355- 2360.

Formula (mg/L)
Boric Acid 143
Calcium Chloride Anhydrous 1359
Citric Acid, Anhydrous 300
Cobalt Nitrate·6H2O 2.45
Cupric Sulfate∙5H2O 3.95
Ferric Ammonium Citrate 300
Magnesium Sulfate∙7H2O 3750
Manganese Chloride∙4H2O 90.5
Na2∙Mg∙EDTA 50
Potassium Phosphate dibasic 2000
Sodium Carbonate Anhydrous 1000
Sodium Molybdate∙2H2O 19.5
Sodium Nitrate 75000
Zinc Sulfate*7H2O 0.222
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