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Introduction:
A carbon source and substrate for ß-glucosidase.
Can be used in mD5A Medium (Prod. # M5516) for the selection of Xanthomonas hortorum. Also used in Cefazoline-Cellobiose-Methionine (CCM) medium as a carbon source for Xanthomonas axonopodis pv. vignicola.Synonyms: 4-O-ß-D-Glucopyranosyl-D-glucose
C1842 Cellobiose
| Synonyms: | 4-O-β-D-Glucopyranosyl-D-glucose |
| CAS: | 528-50-7 |
| Formula: | C12H22O11 |
| Mol. Weight: | 342.30 |
Properties
| Form: | Powder |
| Appearance: | White to Off-White |
| Application: | Microbiology, Seed Testing, Phytopathology |
| Solubility: | Soluble in Water |
| Typical Working Concentration: | Varies with application, should be determined by end user. |
| Storage Temp: | Room Temperature |
| Product# | Product Name | Amount/L |
| P705 | Dipotassium Phosphate, (K2HPO4) | 1.34 g/L |
| P846 | Monopotassium Phosphate (KH2PO4) | 0.4 g/L |
| M150 | Magnesium Sulfate (Anhydrous) (MgSO4) | 0.3 g/L |
| B210 | Boric Acid (H3BO3) | 0.2 g/L |
| A109 | Ammonium Chloride | 1.0 g/L |
| C1842 | Cellobiose | 10.0 g/L |
| M539 | Methionine | 1.0 g/L |
| A111/A296 | Agar | 14.0 g/L |
| C1989/C1796 | *Cycloheximide Powder/ Solution (100 mg/mL) | 0.2 g/L or 2.0 mL/L |
| N/A | *Cefazoline | 0.010 g/L |
| Adjust to pH 7.2 Add aseptically after autoclaving medium and allowing to cool. |
Application Notes
Cellobiose is used in microbiological and phytopathological media as a carbon source for selection of specific microbes. It is used in multiple selection media, including mD5A Medium (Prod # M5516) and CCM medium. The table below is an example of the use of cellobiose as a carbon source in a selection medium.
The semi-selective medium designated as Cefazoline-Cellobiose-Methionine (CCM) medium is intended to select for Xanthomonas axonopodis pv. vignicola and contains the following components per liter (Wydra et al., 2004):
References
Wydra K, G Khatri-Chhetri, A Mavridis, R Sikirou, K Rudolph (2004) A diagnostic medium for the semi-selective
isolation and enumeration of Xanthomonas axonopodis pv. vignicola. European Journal of Plant Pathology,110(10) pp. 991-1001.
| Formula (mg/L) | |
|---|---|
| Boric Acid | 143 |
| Calcium Chloride Anhydrous | 1359 |
| Citric Acid, Anhydrous | 300 |
| Cobalt Nitrate·6H2O | 2.45 |
| Cupric Sulfate∙5H2O | 3.95 |
| Ferric Ammonium Citrate | 300 |
| Magnesium Sulfate∙7H2O | 3750 |
| Manganese Chloride∙4H2O | 90.5 |
| Na2∙Mg∙EDTA | 50 |
| Potassium Phosphate dibasic | 2000 |
| Sodium Carbonate Anhydrous | 1000 |
| Sodium Molybdate∙2H2O | 19.5 |
| Sodium Nitrate | 75000 |
| Zinc Sulfate*7H2O | 0.222 |