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Cellobiose

  • Product ID: C1842

Introduction:

A carbon source and substrate for ß-glucosidase.
Can be used in mD5A Medium (Prod. # M5516) for the selection of Xanthomonas hortorum. Also used in Cefazoline-Cellobiose-Methionine (CCM) medium as a carbon source for Xanthomonas axonopodis pv. vignicola.Synonyms: 4-O-ß-D-Glucopyranosyl-D-glucose

C1842 Cellobiose

Synonyms: 4-O-β-D-Glucopyranosyl-D-glucose
CAS: 528-50-7
Formula: C12H22O11
Mol. Weight: 342.30


Properties

Form: Powder
Appearance: White to Off-White
Application: Microbiology, Seed Testing, Phytopathology
Solubility: Soluble in Water
Typical Working Concentration: Varies with application, should be determined by end user.
Storage Temp: Room Temperature
Product# Product Name Amount/L
P705  Dipotassium Phosphate, (K2HPO4)  1.34 g/L
P846  Monopotassium Phosphate (KH2PO4)  0.4 g/L
M150  Magnesium Sulfate (Anhydrous) (MgSO4) 0.3 g/L
B210 Boric Acid (H3BO3) 0.2 g/L
A109  Ammonium Chloride 1.0 g/L
C1842 Cellobiose  10.0 g/L
M539  Methionine  1.0 g/L
A111/A296 Agar 14.0 g/L
C1989/C1796  *Cycloheximide Powder/ Solution (100 mg/mL) 0.2 g/L or 2.0 mL/L
N/A  *Cefazoline 0.010 g/L
  Adjust to pH 7.2 Add aseptically after autoclaving medium and allowing to cool.  

Application Notes
Cellobiose is used in microbiological and phytopathological media as a carbon source for selection of specific microbes. It is used in multiple selection media, including mD5A Medium (Prod # M5516) and CCM medium. The table below is an example of the use of cellobiose as a carbon source in a selection medium.

The semi-selective medium designated as Cefazoline-Cellobiose-Methionine (CCM) medium is intended to select for Xanthomonas axonopodis pv. vignicola and contains the following components per liter (Wydra et al., 2004):

References
Wydra K, G Khatri-Chhetri, A Mavridis, R Sikirou, K Rudolph (2004) A diagnostic medium for the semi-selective isolation and enumeration of Xanthomonas axonopodis pv. vignicola. European Journal of Plant Pathology,110(10) pp. 991-1001.

Formula (mg/L)
Boric Acid 143
Calcium Chloride Anhydrous 1359
Citric Acid, Anhydrous 300
Cobalt Nitrate·6H2O 2.45
Cupric Sulfate∙5H2O 3.95
Ferric Ammonium Citrate 300
Magnesium Sulfate∙7H2O 3750
Manganese Chloride∙4H2O 90.5
Na2∙Mg∙EDTA 50
Potassium Phosphate dibasic 2000
Sodium Carbonate Anhydrous 1000
Sodium Molybdate∙2H2O 19.5
Sodium Nitrate 75000
Zinc Sulfate*7H2O 0.222
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