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Introduction:
Contains the macro- and micronutrients, and vitamins as described by Chu (1975).
Chu (N6) Medium was developed to promote the initiation, growth, and differentiation of callus from rice pollen cultures. Ammonium Nitrate has been replaced by Ammonium Sulfate. The molar concentration of NH4 is 7.0 mM compared to 20.6mM for MS.
C167 Chu N6 Basal Medium w/ Vitamins
Properties
| Properties | |
|---|---|
| Form: | Powder |
| Appearance: | White to Yellow Powder |
| Application: | Plant Tissue Culture |
| Solubility: | Water |
| Typical Working Concentration: | 3.99g/L |
| Storage Temp: | 2-8°C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insoluble precipitates may form. |
| Other Notes: | Contains the macro- and micronutrients and vitamins as described by Chu et al. (1975). pH = 3.5 – 4.5 |
| Formula (mg/L) | |
|---|---|
| Ammonium Sulfate | 463 |
| Boric Acid | 1.6 |
| Calcium Chloride, Anhydrous | 125.33 |
| Na2 EDTA·2H2O | 37.25 |
| Ferrous Sulfate·7H2O | 27.85 |
| Magnesium Sulfate, Anhydrous | 90.37 |
| Manganese Sulfate·H2O | 3.3 |
| Potassium Iodide | 0.8 |
| Potassium Nitrate | 2830 |
| Potassium Phosphate, Monobasic | 400 |
| Zinc Sulfate·7H2O | 1.5 |
| Glycine (Free Base) | 2 |
| Nicotinic Acid (Free Acid) | 0.5 |
| Pyridoxine·HCl | 0.5 |
| Thiamine·HCl | 1 |
Application Notes
Plant Tissue Culture Tested
Plant species: rice (Oryza sativa) Chu (N6) Medium was developed to promote the initiation, growth, and differentiation of callus from rice pollen cultures.
Ammonium nitrate has been replaced by ammonium sulfate. The molar concentration of NH4 + is 7.0 mM compared to 20.6mM for MS
References
Chu CC, CC Wang, CS Sun, C Hsu, KC Yin, CY Chu and FY Bi. (1975) Scientia Sinic. 18: 659- 668.