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Blaydes Basal Medium

  • Product ID: B514

Introduction:

B514 Blaydes Modified Basal Medium



Properties

Form: Powder
Appearance: Off-white to yellow
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 31.86 g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Contains the macro- and micronutrients, sucrose, and thiamine as described by Blaydes (1966). Plant Tissue Culture Tested.
Formula (mg/L)
Ammonium Nitrate 1000
Boric Acid  1.6
Calcium Nitrate 241.1
Na2EDTA.2H2O 74.5
Ferrous Sulfate.7H2O 55.7
Magnesium Sulfate, Anhydrous 17.1
Manganese Sulfate.H2O 4.4
Potassium Chloride 65
Potassium Iodide  0.8
Potassium Nitrate 100
Potassium Phosphate, Monobasic 300
Zinc Sulfate.7H2O  1.5
Glycine (Free Base) 2
Sucrose 30,000
Thiamine.HCl 0.1

Application Notes
Plant species: Alfalfa
Callus initiation was achieved when the basal medium was supplemented with 1.9 mg/L Kinetin and 8.0 – 19.0 mg/L 2,4-D. Regeneration was achieved when the Kinetin and 2,4-D were omitted and the basal medium was supplemented with 100 mg/L myo-Inositol and 2,000 mg/L yeast extract

References
Blaydes, OF. 1966. Interaction of kinetin and various inhibitors in the growth of soybean tissue. Physiol. Plant 19: 748-753.

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