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BM-2 Terrestrial Orchid Medium without Agar

  • Product ID: B470

Introduction:

Contains the macro-and micronutrients, vitamins, sucrose fructose, agar, and supplements required to culture terrestrial orchids.

B470 BM-2 Terrestrial Orchid Medium w/out Agar



Properties

Properties
Form: Powder
Appearance: White to Cream
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 21.22 g/L
Storage Temp: 2-8° C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Same formulation as B141 with 0.2 mg/L BA
Unadjusted pH = 5.0 – 6.0
BM-2 differs from BM Medium in that it (BM-2) has no inorganic nitrogen and it contains hydrolyzed casein, L-glutamine and BA.
Formula (mg/L)
Boric Acid 10
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Na2 EDTA.2H2O 37.25
Ferrous Sulfate.7H2O 27.85
Magnesium Sulfate, Anhydrous 100
Manganese Sulfate.H2O 25
Molybdic Acid (Sodium Salt) .2H2O 0.25
Potassium Phosphate, Monobasic 300
Zinc Sulfate.7H2O 10
6-Benzylaminopurine (BA) 0.2
D-Biotin 0.05
Casein, Enzymatic Hydrolysate 500
Folic Acid 0.5
L-Glutamine 100
Glycine (Free Base) 2
myo-Inositol 100
Nicotinic Acid (Free Acid) 5
Pyridoxine.HCl 0.5
Sucrose 20,000
Thiamine.HCl 0.5

Application Notes
Plant Tissue Culture Tested
Well suited for the terrestrial orchids Paphiopedilum and Phragmipedium.

References
According to "The Orchid Seedbank Project" this was originally cited by Van Waes, 1984. In vitro studie van de kiemingsfysiologie van Westeuropese orchideeën. Thesis. Rijkuniversiteit Gent.

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