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Bold's Basal Medium (BBM)

  • Product ID: B1675

Introduction:

Bold's Basal Medium (BBM) is a freshwater algae medium that has been used to grow a variety of green algal cultures (e.g. Trichosarcina, Chlorococcum, and Chlorella) without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965). The predominantly inorganic nature of this medium facilitates itself as an axenic-culture maintenance medium (Nichols and Bold, 1965)
Requires 1 mL/L Sulfuric Acid, 0.1% (v/v) (Product # S7664).

B1675 Bold's Basal Medium

Synonyms: BBM


Properties

Properties
Form: Powder
Appearance: Off-White to Gray/Green
Application: Freshwater algal culture
Solubility: Soluble in Water
Typical Working Concentration: 0.705 g/L
Storage Temp: 2-8ºC
Storage Temp of Stock Solution: 2-8ºC
Other Notes: In order to conform with the original reference, 1.0 mL of 0.1% Sulfuric Acid Solution should be added per liter of this medium when prepared at a 1x concentration.
Product or solutions may develop a purple tint over time.
Formula (mg/L)
Boric Acid 11.42
Manganese Chloride.4H2O 1.44
Calcium Chloride, Anhydrous 18.87
Potassium Hydroxide 31
Cobalt Nitrate.6H2O 0.49
Potassium Phosphate, Dibasic 75
Cupric Sulfate.5H2O 1.57
Potassium Phosphate, Monobasic 175
EDTA, Disodium Salt 63.61
Sodium Chloride 25
Ferrous Sulfate.7H2O 4.98
Sodium Molybdate 1.19
Magnesium Sulfate, Anhydrous 36.63
Sodium Nitrate 250
Zinc Sulfate.7H2O 8.82

Application Notes
Bold's Basal Medium (BBM) is a freshwater algae medium that has been used to grow a variety of green algal cultures (e.g. Trichosarcina, Chlorococcum, and Chlorella) without the need for soil-extract or vitamins (Brown et al., 1964; Nichols and Bold, 1965). The predominantly inorganic nature of this medium facilitates itself as an axenic-culture maintenance medium (Nichols and Bold, 1965).

Other algae species commonly grown in Bold's Basal Medium:
Zyngogonium ericetorum (Stancheva et al., 2014)

Media Preparation:
The standard medium is prepared as follows: Add 1.0 mL of 0.1% Sulfuric Acid solution to 1 liter of DI water along with 0.705 g of B1675. The final solution pH is adjusted to 6.6 +/- 0.1 with KOH (Stein, 1973).

References
Brown, R.M., Larson, D.A., and H.C. Bold. (1964) Science 143(3606), 583-585.
Nichols H.W., and H.C. Bold (1965) J. Phycology 1, 34-38.
Stancheva, R., Hall, J. D., Herburger, K., Lewis, L. A., McCourt, R. M., Sheath, R. G., & Holzinger, A. (2014).
Phylogenetic position of Zygogonium ericetorum (Zygnematophyceae, Charophyta) from a high alpine habitat and ultrastructural characterization of unusual aplanospores. Journal of Phycology, 50(5), 790-803.
Stein J. (1973) Handbook of Phycological methods. Culture Methods and Growth Measurements. Cambridge University Press. 448 pp.

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