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BM-2 Terrestrial Orchid Medium with Agar

  • Product ID: B142

Introduction:

Contains the macro- and micronutrients, vitamins, and plant growth regulators required to culture orchids. Contains 0.2 mg/L 6-benzylaminopurine (BA).

B142 BM-2 Terrestrial Orchid Medium



Properties

Form: Powder
Appearance: White to Cream
Application: Plant Tissue Culture
Solubility: Fully Soluble in Hot Water, Partially Soluble in Cold Water
Typical Working Concentration: 27.22 g/L
Storage Temp: 2-8° C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Other Notes: Same formulation as B141 with 0.2 mg/L BA; contains agar.
Unadjusted pH = 5.0 – 6.0
BM-2 differs from BM Medium in that it (BM-2) has no inorganic nitrogen and it contains hydrolyzed casein, L-glutamine, and 6-Benzylaminopurine (BA).
Formula (mg/L)
Boric Acid 10
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Na2 EDTA.2H2O 37.25
Ferrous Sulfate.7H2O 27.85
Magnesium Sulfate, Anhydrous 100
Manganese Sulfate.H2O 25
Molybdic Acid (Sodium Salt) .2H2O 0.25
Potassium Phosphate, Monobasic 300
Zinc Sulfate.7H2O 10
Agar 6,000
6-Benzylaminopurine (BA) 0.2
D-Biotin 0.05
Casein, Enzymatic Hydrolysate 500
Folic Acid 0.5
L-Glutamine 100
Glycine (Free Base) 2
myo-Inositol 100
Nicotinic Acid (Free Acid) 5
Pyridoxine.HCl 0.5
Sucrose 20,000
Thiamine.HCl 0.5

Application Notes
Plant Tissue Culture Tested
Especially suited for terrestrial orchids Paphiopedilum and Phragmipedium.

References
According to "The Orchid Seedbank Project" this was originally cited by Van Waes, 1984. In vitro studie van de kiemingsfysiologie van Westeuropese orchideeën. Thesis. Rijkuniversiteit Gent.

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