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Introduction:
BG-11 Medium is a freshwater growth medium for cyanobacteria (e.g., Synechocytis spp. PCC 6803). This is a 50x concentrated solution.
B1411 BG-11 Medium
| Synonyms: | Blue-Green (BG) Medium |
Properties
| Form: | Liquid |
| Appearance: | Light Yellow to Yellow |
| Application: | Cyanobacteria Media |
| Solubility: | Miscible with Water |
| Typical Working Concentration: | 20.00 mL/L |
| Storage Temp: | 2-8ºC |
| Storage Temp of Stock Solution: | 2-6ºC |
| Biological Assasy: | Biologically tested with Synechocystis sp. PCC 6803. Solution pH is approx. 4.2 +/- 0.5 |
| Other Notes: | It is suggested not to autoclave BG-11, 50x concentrate solution as precipitation may result. |
| Formula (mg/L) | |
|---|---|
| Boric Acid | 143 |
| Calcium Chloride Anhydrous | 1359 |
| Citric Acid, Anhydrous | 300 |
| Cobalt Nitrate·6H2O | 2.45 |
| Cupric Sulfate∙5H2O | 3.95 |
| Ferric Ammonium Citrate | 300 |
| Magnesium Sulfate∙7H2O | 3750 |
| Manganese Chloride∙4H2O | 90.5 |
| Na2∙Mg∙EDTA | 50 |
| Potassium Phosphate dibasic | 2000 |
| Sodium Carbonate Anhydrous | 1000 |
| Sodium Molybdate∙2H2O | 19.5 |
| Sodium Nitrate | 75000 |
| Zinc Sulfate*7H2O | 0.222 |
Application Notes
BG-11 is generally a freshwater growth medium for culturing Cyanobacteria such as Synechocystis sp. PCC 6803.
HEPES - H326 (Akira et al. 1996) or TES (Xu et al. 1994) can be added to buffer the pH. After dissolving the powder medium, adjust to pH 8.0 with KOH or NaOH, and autoclave.
20 mM Hepes (H326) was used during biological testing of this product with Synechocystis sp. PCC 6803.
References
Akira, K., K. Lee, H. Fukuzawa, K. Ohyama, and T. Ogawa. (1996) cemA homologue essential to CO2 transport in the cynanobacterium Synechocystis PCC 6803. Proceedings of the National Academy of Sciences. 93:4006-4010.
Rippka, R., J. Deruelles, J. B. Waterbury, M. Herdman, and R. Y. Stanier (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Journal of General Microbiology 111:1-61.
Stanier, R.Y., R. Kunisawa, M. Mandel, and G. Cohen-Bazire. (1971) "Purification and Properties of Unicellular Blue-Green Algae (Order Chroococcales)." Bacteriological Reviews 35(2):171-205.
Xu, H., D. Vavilin, C. Funk, and W. Vermaas. "Multiple Deletions of Small-Cab-like Proteins.