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BG-11 (Blue-green) Medium Solution 50x

  • Product ID: B1411

Introduction:

BG-11 Medium is a freshwater growth medium for cyanobacteria (e.g., Synechocytis spp. PCC 6803). This is a 50x concentrated solution.

B1411 BG-11 Medium

Synonyms: Blue-Green (BG) Medium


Properties

Form: Liquid
Appearance: Light Yellow to Yellow
Application: Cyanobacteria Media
Solubility: Miscible with Water
Typical Working Concentration: 20.00 mL/L
Storage Temp: 2-8ºC
Storage Temp of Stock Solution: 2-6ºC
Biological Assasy: Biologically tested with Synechocystis sp. PCC 6803.
Solution pH is approx. 4.2 +/- 0.5
Other Notes: It is suggested not to autoclave BG-11, 50x concentrate solution as precipitation may result.
Formula (mg/L)
Boric Acid 143
Calcium Chloride Anhydrous 1359
Citric Acid, Anhydrous 300
Cobalt Nitrate·6H2O 2.45
Cupric Sulfate∙5H2O 3.95
Ferric Ammonium Citrate 300
Magnesium Sulfate∙7H2O 3750
Manganese Chloride∙4H2O 90.5
Na2∙Mg∙EDTA 50
Potassium Phosphate dibasic 2000
Sodium Carbonate Anhydrous 1000
Sodium Molybdate∙2H2O 19.5
Sodium Nitrate 75000
Zinc Sulfate*7H2O 0.222

Application Notes
BG-11 is generally a freshwater growth medium for culturing Cyanobacteria such as Synechocystis sp. PCC 6803.

HEPES - H326 (Akira et al. 1996) or TES (Xu et al. 1994) can be added to buffer the pH. After dissolving the powder medium, adjust to pH 8.0 with KOH or NaOH, and autoclave.

20 mM Hepes (H326) was used during biological testing of this product with Synechocystis sp. PCC 6803.

References
Akira, K., K. Lee, H. Fukuzawa, K. Ohyama, and T. Ogawa. (1996) cemA homologue essential to CO2 transport in the cynanobacterium Synechocystis PCC 6803. Proceedings of the National Academy of Sciences. 93:4006-4010.
Rippka, R., J. Deruelles, J. B. Waterbury, M. Herdman, and R. Y. Stanier (1979) Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Journal of General Microbiology 111:1-61.
Stanier, R.Y., R. Kunisawa, M. Mandel, and G. Cohen-Bazire. (1971) "Purification and Properties of Unicellular Blue-Green Algae (Order Chroococcales)." Bacteriological Reviews 35(2):171-205.
Xu, H., D. Vavilin, C. Funk, and W. Vermaas. "Multiple Deletions of Small-Cab-like Proteins.

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