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Broadleaf Tree Basal Medium

  • Product ID: B1396

Introduction:

Contains 0.2 mg/L 6-Benzylaminopurine (BA, product B800).
Contains the macro- and micronutrients, vitamins, and supplements required to culture orchids.

B1396 Broadleaf Tree Basal Medium



Properties

Form: Powder
Appearance: White to Yellow
Application: Plant Tissue Culture
Solubility: Soluble in Water
Typical Working Concentration: 1.21 g/L
Storage Temp: 2-8°C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insoluble precipitates may form.
Formula (mg/L)
Ammonium Nitrate 82.5
 Manganese Sulfate, Monohydrate  4.225
 Ammonium Sulfate  120
 Molybdic Acid, Disodium, Dihydrate  0.063
 Boric Acid  1.55
 Potassium Iodide 0.038
 Calcium Chloride 16.6
Potassium Nitrate  95
 Calcium Nitrate 222
Potassium Phosphate, Monobasic 85
 Cobalt Chloride, Hexahydrate 0.005
 Potassium Sulfate  430
 Cupric Sulfate, Pentahydrate 0.063
 Zinc Sulfate, Heptahydrate 2.15
 EDTA, Disodium, Dihydrate 9.325
 myo-Inositol  50
Ferrous Sulfate, Heptahydrate 6.95
Nicotinic Acid 0.25
 Magnesium Sulfate, Anhydrous  90

Application Notes
Plant tissue culture tested. Plant Species: oak (Quereus), linden (Tilia), and related broadleaf trees. Contains the micro- and macro-nutrients and vitamins described by Chalupa (1984).

References
Chalupa, 1984. Biologia Plantarum. 26(5):374-377.

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