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BM-1 Terrestrial Orchid Medium without Agar

  • Product ID: B138

Introduction:

hydrolyzed casein. This medium is used to identify contamination in plant tissue cultures that may otherwise go unnoticed. This medium is not intended to support the growth of plant tissues, therefore tissue inoculation is not suggested. Simply stab plant tissues into medium or swipe across the surface of the medium.
Formulation as described by Viss & Driver (1991).

B138 BM-1 Terrestrial Orchid Medium w/out Agar



Properties

Form: Powder
Appearance: White to Yellow Powder
Application: Orchid Culture
Solubility: Water
Typical Working Concentration: 21.22 g/L
Storage Temp: 2-8° C
Storage Temp of Stock Solution: Preparation of concentrated solutions is not recommended as insolubles may form.
Other Notes: Same formulation as B141 without agar.
Formula (mg/L)
Boric Acid 10
Cobalt Chloride.6H2O 0.025
Cupric Sulfate.5H2O 0.025
Na2 EDTA.2H2O 37.25
Ferrous Sulfate.7H2O 27.85
Magnesium Sulfate, Anhydrous 100
Manganese Sulfate.H2O 25
Molybdic Acid (Sodium Salt) .2H2O 0.25
Potassium Phosphate, Monobasic 300
Zinc Sulfate.7H2O 10
D-Biotin 0.05
Casein, Enzymatic Hydrolysate 500
Folic Acid 0.5
L-Glutamine 100
Glycine (Free Base) 2
myo-Inositol 100
Nicotinic Acid (Free Acid) 5
Pyridoxine.HCl 0.5
Sucrose 20,000
Thiamine.HCl 0.5

Application Notes
Plant Tissue Culture Tested
Especially suited for terrestrial orchids, Paphiopedilum, and Phragmipedium.
Seed germination may be enhanced by the addition of 50 mL/L Coconut Water (Prod. No. C195).
Consult the Orchid Media Selection & Use Guide for additional information; this can be downloaded from the Technical Info page on the PhytoTechnology Labs web site.

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