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Introduction:
hydrolyzed casein. This medium is used to identify contamination in plant tissue cultures that may otherwise go unnoticed. This medium is not intended to support the growth of plant tissues, therefore tissue inoculation is not suggested. Simply stab plant tissues into medium or swipe across the surface of the medium.
Formulation as described by Viss & Driver (1991).
B138 BM-1 Terrestrial Orchid Medium w/out Agar
Properties
| Form: | Powder |
| Appearance: | White to Yellow Powder |
| Application: | Orchid Culture |
| Solubility: | Water |
| Typical Working Concentration: | 21.22 g/L |
| Storage Temp: | 2-8° C |
| Storage Temp of Stock Solution: | Preparation of concentrated solutions is not recommended as insolubles may form. |
| Other Notes: | Same formulation as B141 without agar. |
| Formula (mg/L) | |
|---|---|
| Boric Acid | 10 |
| Cobalt Chloride.6H2O | 0.025 |
| Cupric Sulfate.5H2O | 0.025 |
| Na2 EDTA.2H2O | 37.25 |
| Ferrous Sulfate.7H2O | 27.85 |
| Magnesium Sulfate, Anhydrous | 100 |
| Manganese Sulfate.H2O | 25 |
| Molybdic Acid (Sodium Salt) .2H2O | 0.25 |
| Potassium Phosphate, Monobasic | 300 |
| Zinc Sulfate.7H2O | 10 |
| D-Biotin | 0.05 |
| Casein, Enzymatic Hydrolysate | 500 |
| Folic Acid | 0.5 |
| L-Glutamine | 100 |
| Glycine (Free Base) | 2 |
| myo-Inositol | 100 |
| Nicotinic Acid (Free Acid) | 5 |
| Pyridoxine.HCl | 0.5 |
| Sucrose | 20,000 |
| Thiamine.HCl | 0.5 |
Application Notes
Plant Tissue Culture Tested
Especially suited for terrestrial orchids, Paphiopedilum, and Phragmipedium.
Seed germination may be enhanced by the addition of 50 mL/L Coconut Water (Prod. No. C195).
Consult the Orchid Media Selection & Use Guide for additional information; this can be downloaded from the Technical Info page on the PhytoTechnology Labs web site.