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Bacitracin Zinc

  • Product ID: B132

Introduction:

Bacitracin Zinc interferes with cell well biosynthesis in gram-positive bacteria.
USP Grade.

B132 Bacitracin Zinc

Synonym: N/A
CAS: 1405-89-6
Formula: C66H101N17O16SZn
Molecular Wt: 1486.07


Properties

Form: Powder
Appearance: Cream to Tan Powder
Application: Plant Tissue Culture Antibiotic
Solubility: Soluble in Water:5.1 mg/mL (28° C), methanol at 6.55 mg/mL, ethanol at2.0 mg/mL, and isopropanol at 0.16 mg/mL per the Merck Index. PhytoTechnology Labs tests solubility at 1.0 mg/mL in DMSO.
Storage Temp: 2 to 8°C
Storage Temp of Stock Solution: The preparation of stock solutions is not recommended. Bacitracin has been observed to lose potency in solution (Merck Index). For optimal potency solutions should be prepared fresh.
Other Notes: Minimum Potency: 65 IU/mg (see Certificate of Analysis for lot specificactivity)

Application Notes
Bacitracin interferes with cell wall biosynthesis in Gram -positive bacteria by inhibiting the dephosphorylation of lipid pyrophosphate . It is also active in some Gram -negative organisms although most are resistant.2

Bacitracin Zn is a mixture of bacitracin A, B1, B2, B3, and Zn. It has been reported that the bacteriostatic activity of bacitracin increases by twofold when Z n was added at 1 x 10 -5 M and 10- fold at 1 x 10 -4 M. Additionally, the presence of Zn ion helps enhance thermal and storage stability of the bacitracin.3

It has been reported that bacitracin's minimum inhibitory concentration for Clostridium perfringens is 0.93 µg/mL . 4 Please Note: It is the sole responsibility of the purchaser to determine the appropriateness of this product for the specific plants that are being cultured and applications that are being used.


References
1. Merck 13, 938
2. Martindale: The Complete D rug Reference , 3 5th ed., Paul S. Blake , Ed. (Royal Pharmaceutical Society, 2007), p. 187.
3. Smith, J.L., and E.D. Weinberg. 1962. Mechanisms of Antibacterial Action of Bacitracin. J. Gen. Microbiol. 28:559- 569.
4. Richez P., Richard A., Cornez B. and Vancraeynest D. "Susceptibility, resistance and antibiotic profile of bacitracin against clostridium perfringens strains isolated during clinical outbreaks of epizootic rabbit enteropathy." 9th World Rabbit Congress - June 10- 13, 20 08 - Verona, Italy.

Formula (mg/L)
Boric Acid 143
Calcium Chloride Anhydrous 1359
Citric Acid, Anhydrous 300
Cobalt Nitrate·6H2O 2.45
Cupric Sulfate∙5H2O 3.95
Ferric Ammonium Citrate 300
Magnesium Sulfate∙7H2O 3750
Manganese Chloride∙4H2O 90.5
Na2∙Mg∙EDTA 50
Potassium Phosphate dibasic 2000
Sodium Carbonate Anhydrous 1000
Sodium Molybdate∙2H2O 19.5
Sodium Nitrate 75000
Zinc Sulfate*7H2O 0.222
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