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Introduction:
Bacitracin Zinc interferes with cell well biosynthesis in gram-positive bacteria.
USP Grade.
B132 Bacitracin Zinc
| Synonym: | N/A |
| CAS: | 1405-89-6 |
| Formula: | C66H101N17O16SZn |
| Molecular Wt: | 1486.07 |
Properties
| Form: | Powder |
| Appearance: | Cream to Tan Powder |
| Application: | Plant Tissue Culture Antibiotic |
| Solubility: | Soluble in Water:5.1 mg/mL (28° C), methanol at 6.55 mg/mL, ethanol at2.0 mg/mL, and isopropanol at 0.16 mg/mL per the Merck Index. PhytoTechnology Labs tests solubility at 1.0 mg/mL in DMSO. |
| Storage Temp: | 2 to 8°C |
| Storage Temp of Stock Solution: | The preparation of stock solutions is not recommended. Bacitracin has been observed to lose potency in solution (Merck Index). For optimal potency solutions should be prepared fresh. |
| Other Notes: | Minimum Potency: 65 IU/mg (see Certificate of Analysis for lot specificactivity) |
Application Notes
Bacitracin interferes with cell wall biosynthesis in Gram
-positive bacteria
by inhibiting the dephosphorylation of lipid
pyrophosphate
. It is also active in some Gram
-negative organisms although most are resistant.2
Bacitracin Zn is a mixture of bacitracin A, B1, B2, B3, and Zn. It has been reported that the bacteriostatic activity of
bacitracin increases by twofold when Z
n was added
at
1 x 10
-5
M and 10-
fold
at 1 x 10
-4
M. Additionally, the
presence of
Zn
ion
helps enhance thermal and storage stability of the bacitracin.3
It has been reported that bacitracin's minimum inhibitory concentration for
Clostridium perfringens
is 0.93 µg/mL
.
4
Please Note: It is the sole responsibility of the purchaser to determine the appropriateness of this product for the
specific plants that are being cultured and applications that are being used.
References
1. Merck 13, 938
2.
Martindale: The Complete D
rug Reference
, 3
5th ed.,
Paul S. Blake
, Ed. (Royal Pharmaceutical
Society,
2007), p. 187.
3. Smith, J.L., and E.D. Weinberg. 1962. Mechanisms of Antibacterial Action of Bacitracin.
J. Gen. Microbiol.
28:559-
569.
4. Richez P., Richard A., Cornez B. and Vancraeynest D. "Susceptibility, resistance and antibiotic
profile of bacitracin against
clostridium perfringens
strains isolated during clinical outbreaks of epizootic
rabbit enteropathy."
9th World Rabbit Congress -
June 10-
13, 20
08
- Verona, Italy.
| Formula (mg/L) | |
|---|---|
| Boric Acid | 143 |
| Calcium Chloride Anhydrous | 1359 |
| Citric Acid, Anhydrous | 300 |
| Cobalt Nitrate·6H2O | 2.45 |
| Cupric Sulfate∙5H2O | 3.95 |
| Ferric Ammonium Citrate | 300 |
| Magnesium Sulfate∙7H2O | 3750 |
| Manganese Chloride∙4H2O | 90.5 |
| Na2∙Mg∙EDTA | 50 |
| Potassium Phosphate dibasic | 2000 |
| Sodium Carbonate Anhydrous | 1000 |
| Sodium Molybdate∙2H2O | 19.5 |
| Sodium Nitrate | 75000 |
| Zinc Sulfate*7H2O | 0.222 |