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6-Benzylaminopurine (BA) Solution (1 mg/mL)

  • Product ID: B130

Introduction:

6-Benzylaminopurine is the most widely used adenine-based cytokinin.
Synonyms: BA; N6-Benzyladenine

B130 6-Benzylaminopurine Solution (1 mg/mL)

Synonyms: BA; N6-Benzyladenine
CAS: 1214-39-7
Formula: C12H11N5
Mol. Weight: 225.3


Properties

Form: Liquid
Appearance: Colorless, Clear Liquid
Application: Plant Growth Regulator; Cytokinin
Solubility: Miscible with Water
Storage Temp: 2 to 8 °C
Typical Working Concentration: Varies by application. Concentration should be determined by end user
Other Notes: Plant Tissue Culture Tested; For Research Use only

Application Notes
6-Benzylaminopurine(BA) is one of the most popular cytokinins used to stimulate in vitro shoot development. It is often used in combination with an auxin, e.g., Indole-3-acetic acid (IAA), Napthaleneacetic acid (NAA), Indole-3- butyric acid (IBA).

BA is active across a broad range of plant species. Typical working concentration of BA is between 0.1 – 5.0 mg/L. It has been reported that 5-10 mg/L of BA is an optimal range for shoot multiplication of banana culture1 ; while 1.0 and 2.0 mg/L of BA have been used for blackberry culture. 2 At PhytoTechnology Laboratories® most cultures (e.g., Achimenes, African violet, ajuga, begonia, hosta, syngonium, etc.) are maintained on MS medium supplemented with 1 mg/L of BA and 0.025 mg/L of NAA.

PhytoTechnology Laboratories® also carried 6-Benzylaminopurine Powder, Product No. B800.Please Note: While PhytoTechnology Laboratories™ tests each lot of this product with two or more plant cell/ tissue culture lines, it is the sole responsibility of the purchaser to determine the appropriateness of this product for the specific plants that are being cultured and applications that are being used.


References
1. Vuylsteke, D.R. 1998. Shoot-tip culture for the propagation, conservation, and distribution of Musa germplasm. International Institute of Tropical Agriculture, Ibadan, Nigeria. 82 pp.
2. Bobrowski, Vera L., Mello-Farias, Paulo C., and Peters, Jose A. 1996. Micropropagation of blackberries (Rubus sp.) cultivars. Rev. Bras. De Agrociencia. 2(1):17-20.

Formula (mg/L)
Boric Acid 143
Calcium Chloride Anhydrous 1359
Citric Acid, Anhydrous 300
Cobalt Nitrate·6H2O 2.45
Cupric Sulfate∙5H2O 3.95
Ferric Ammonium Citrate 300
Magnesium Sulfate∙7H2O 3750
Manganese Chloride∙4H2O 90.5
Na2∙Mg∙EDTA 50
Potassium Phosphate dibasic 2000
Sodium Carbonate Anhydrous 1000
Sodium Molybdate∙2H2O 19.5
Sodium Nitrate 75000
Zinc Sulfate*7H2O 0.222
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