| ISBN |
0-9673049-0-3 |
| Year |
2006 |
| Material |
Paperback |
| Pages |
134 |
| Storage Temp |
Room Temperature |
| UPC / SKU |
B019 |
| Storage Temp. |
Room Temperature |
| Tariff Code |
4901.99.0050 |
| Risk Info (R) |
22-36/37/38 |
| Safety Info (S) |
22-24/25-36 |
Component
B019 Book, Asymbiotic Technique of Orchid Seed Germination
About the Book:
Asymbiotic Technique of Orchid Seed Germination is the most comprehensive text to date on the subject of growing orchids from seed.
Designed to accommodate everyone from the novice to those working in established orchid labs, Orchid Seed Germination provides valuable information on all phases of growing orchids from seed. From disinfection to deflasking, this manual provides references, in-depth information, and the very latest on the seed propagation of orchids.
The author is the director of the Orchid Seedbank Project, a research and conservation effort to help propagate and conserve orchids.
Contents:
Chapter 1: The orchid seed: Germination
Orchid mycorrhiza
Chapter 2: A brief history of orchids from seed
History
Chapter 3: The basics
Orchid seed production (green capsule, pollination, pollination of miniature flowers, pollination of vanilla, pollen storage, pollen viability)
Orchid seed harvesting
Orchid seed storage (humidity, hydrates, relative humidity, measuring humidity, constant humidity solutions, longevity, short-lived seed, rehydration)
Handling and shipping of orchid seed (static controls, laws)
Lab basics (chemical safety, glassware, lab equipment, pH, buffers, suitable pH for germination of orchid seed)
Media, sterilization, and bacteriology 101 (chemical sterilization, thermal sterilization, filtration sterilization, denaturation, UV light sterilization, radiation sterilization, microwaves, introductory microbiology)
Preparation of the media (types of media, fertilizer-based media, improvised media, formulae for expedient orchid seed germination, stock solutions, stock solution preparation, aseptic sowing area, flasks)
Chapter 4: Technique
Preparation of the sowing area (glass sheet technique, glove boxes, laminar flow hoods, hands, bleach vs. alcohol)
Disinfection of seed (batch washing, filtration, dropwise, decanting, rapidfire, chlorine gas sterilization, floating seed, disinfection times, vacuum treatment, documentation)
Green capsule technique (technique)
Variations on a theme (use of steam during flasking, techniques for introducing seed, OSP technique, small-volume flasks, sterile area alternatives, lid seals, miscellaneous)
Chapter 5: Post-sowing
Dealing with contamination problems (aggressive efforts, source of contamination, treating plugs, sealing flasks, mites, vacuum treatment)
Care of the sown flasks (dry flasks, root growth, disposal of flasks and conditioning)
Replate
Deflasking and seedling care
Chapter 6: Advanced techniques
Media modifications (charcoal, water, iron, sugars, vitamins, nitrogen, inositol, amino-acid based media (entire section for cyps!), making your own media, fruit juices and other extracts, anticontaminants in media, nutrient deficiencies)
Specific genera (terrestrial orchids)
Making your own glove box
Manufacturing baffle ports for a glove box
New directions in seed disinfection (thermal, gas, peroxides, hypochlorites, duration of exposure with hypochlorites, Virkon S, DCCA, outdated techniques)
Practicing with inert compounds
Alternative gelling agents (PhytaGel, kappa carrageenan, membrane rafts, capillary media, super absorbent polymers)
Viability testing and seed analysis (microscopic examination, seed types, chemical staining, photomicrography, floating seed)
Appendix I:
Where to start (media, flasking area, seeds, sowing, easy starts)
Appendix II:
Improvised home flasking
Appendix III:
Sources, conversions, additional reading, trademarks, about the author, about the OSP, how it works)
