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Introduction:
Agar, Plant TC Micropropagation Grade from Gelidium sp.
A296 Agar, Plant TC Micropropagation Grade - Gelidium
| Synonyms: | Agar-Agar, Gum Agar |
| CAS: | 9002-18-0 |
Properties
| Form: | Powder |
| Appearance: | Cream to Tan |
| Application: | Gelling Agent |
| Solubility: | Partially Soluble in Cold Water; Soluble in Boiling Water |
| Typical Working Concentration: | 6 to 10 grams per liter |
| Storage Temp: | Room Temperature |
| Other Notes: | Gel Strength: 700-950 g/cm2 Ash Content: <6% Plant Tissue Culture Tested |
Application Notes
Agar is produced from a family of red seaweeds (Rhodophycae) primarily from two genera, Gelidium and Gracilaria, and is a mixture of agarose and agaropectin. Product A296 is produced from Gelidium typically has higher gel strength than those produced from Gracilaria. 2
Agar is by far the most common gelling agent used in plant tissue culture. It is used at a wide range of concentrations from 6 g/L for a slightly firm gel to 10 g/L for a brick-like gel; however, 6-8 g/L is the more commonly used range.
Product No. A296 is a popular gelling agent for orchid culture; also popular in microbiological/ bacteriological applications due to its clarity and light color.
PhytoTechnology Laboratories® also carries high purity grade agar, Product No. A175, as well as agar produced from Gracilaria, Product No. A111.
Please Note: While PhytoTechnology Laboratories® tests each lot of this product with two or more plant cell/ tissue culture lines, it is the sole responsibility of the purchaser to determine the appropriateness of this product for the specific plants that are being cultured and applications that are being used.
References
1. Merck 13, 184
2. McHugh, D.J. (2003). A guide to the seaweed industry. FAO Fisheries Technical Paper 441, Food and Agriculture Organization of the United Nations, Rome.
| Formula (mg/L) | |
|---|---|
| Boric Acid | 143 |
| Calcium Chloride Anhydrous | 1359 |
| Citric Acid, Anhydrous | 300 |
| Cobalt Nitrate·6H2O | 2.45 |
| Cupric Sulfate∙5H2O | 3.95 |
| Ferric Ammonium Citrate | 300 |
| Magnesium Sulfate∙7H2O | 3750 |
| Manganese Chloride∙4H2O | 90.5 |
| Na2∙Mg∙EDTA | 50 |
| Potassium Phosphate dibasic | 2000 |
| Sodium Carbonate Anhydrous | 1000 |
| Sodium Molybdate∙2H2O | 19.5 |
| Sodium Nitrate | 75000 |
| Zinc Sulfate*7H2O | 0.222 |