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Introduction:
Agar, Plant TC Micropropagation Grade from Gracilaria sp.This is a higher gel-strength tissue-culture grade agar over A296.
A111 Agar, Plant TC Micropropagation Grade - Gracilaria
| Synonyms: | Agar-Agar, Gum Agar |
| CAS: | 9002-18-0 |
Properties
| Form: | Powder |
| Appearance: | Off-white to Cream |
| Application: | Gelling Agent |
| Solubility: | Partially Soluble in Cold Water; Soluble in Boiling Water |
| Typical Working Concentration: | 6 to 10 grams per liter |
| Storage Temp: | Room Temperature |
| Other Notes: | Gel Strength: ≥900 g/cm2 Ash Content: <6% Plant Tissue Culture Tested |
Application Notes
Agar is produced from a family of red seaweeds (Rhodophycae) primarily from two genera, Gelidium and Gracilaria. It is a mixture of agarose and agaropectin. Product A111 is produced from Gracilaria which has lower gel strength than those from Gelidium. However, in the 1950’s it was discovered that pre-treatment of the seaweed with alkali before extraction lowered the yield but gave an agar with higher gel strength.2
Agar is by far the most common gelling agent used in plant tissue culture. It is used at a wide range of concentrations from 6 g/L for a slightly firm gel to 10 g/L for a brick-like gel; however, 6-8 g/L is the more commonly used range.
PhytoTechnology Laboratories® also carries agar produced from Gelidium, Product No. A296, as well as high purity grade agar, Product No. A175.
Please Note: While PhytoTechnology Laboratories® tests each lot of this product with two or more plant cell/ tissue culture lines, it is the sole responsibility of the purchaser to determine the appropriateness of this product for the specific plants that are being cultured and applications that are being used.
References
1.Merck 13, 184
2.McHugh, D.J. (2003). A guide to the seaweed industry. FAO Fisheries Technical Paper 441, Food and Agriculture Organization of the United Nations, Rome
| Formula (mg/L) | |
|---|---|
| Boric Acid | 143 |
| Calcium Chloride Anhydrous | 1359 |
| Citric Acid, Anhydrous | 300 |
| Cobalt Nitrate·6H2O | 2.45 |
| Cupric Sulfate∙5H2O | 3.95 |
| Ferric Ammonium Citrate | 300 |
| Magnesium Sulfate∙7H2O | 3750 |
| Manganese Chloride∙4H2O | 90.5 |
| Na2∙Mg∙EDTA | 50 |
| Potassium Phosphate dibasic | 2000 |
| Sodium Carbonate Anhydrous | 1000 |
| Sodium Molybdate∙2H2O | 19.5 |
| Sodium Nitrate | 75000 |
| Zinc Sulfate*7H2O | 0.222 |