Enquiry E-CATALOGUE

Acetosyringone Solution (100 mM) in DMSO

  • Product ID: A1104

Introduction:

Acetosyringone is a naturally occurring compound secreted from wounded dicot plant tissues and is a chemotractant for Agrobacterium sp. It is generally used at 5 uM to 200 uM to enhance transformation in monocots.Acetosyringone should NOT be autoclaved. This is a sterile solution of Acetosyringone in DMSO.

Synonym: 3',5'-Dimethyoxy-4'-hydroxyacetophenone

A1104 Acetosyringone 100 mM Solution

Synonyms: 4-Hydroxy-3’,5’-dimethoxyacetophenone
CAS: 2478-38-8
Formula: C10H12O4
Mol. Weight: 196.20


Properties

Form: Liquid
Appearance: Off-white to Tan
Application: Molecular Biology
Solubility: Miscible in Water
Typical Working Concentration: 50 µM to 200 µM
Storage Temp: -20 °C
Storage Temp of Stock Solution: N/A
Other Notes: Plant Tissue Culture Tested

Application Notes
This material is sensitive to temperature and should be added after autoclaved media has cooled slightly. Acetosyringone is a naturally occurring compound secreted from wounded plant tissues. It is used to increase the rate of transformation when used at 50 µM to 200 µM. (Sheikholeslam & Weeks, 1987).

References
Sheikholeslam, SN & DP Weeks. 1987. Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens. Plant Molecular Biology 8:291- 298.

Formula (mg/L)
Boric Acid 143
Calcium Chloride Anhydrous 1359
Citric Acid, Anhydrous 300
Cobalt Nitrate·6H2O 2.45
Cupric Sulfate∙5H2O 3.95
Ferric Ammonium Citrate 300
Magnesium Sulfate∙7H2O 3750
Manganese Chloride∙4H2O 90.5
Na2∙Mg∙EDTA 50
Potassium Phosphate dibasic 2000
Sodium Carbonate Anhydrous 1000
Sodium Molybdate∙2H2O 19.5
Sodium Nitrate 75000
Zinc Sulfate*7H2O 0.222
Life Technologies (India) Pvt Ltd. 

306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India)
Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444,
Mobile# +91-98105-21400, Toll Free# 1800-120-2434
Email# customerservice@lifetechindia.com