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L-Ascorbic Acid

  • Product ID: A106

Introduction:

L-Ascorbic acid is a vitamin and antioxidant that are often supplemented to plant tissue culture media to inhibit tissue browning.
Synonyms: 3-oxo-L-gulofuranolactone; Vitamin C

A106 L-Ascorbic Acid

Synonyms: 3-Oxo-L-gulofuranolactone, Vitamin C
CAS: 50-81-7
Formula: C6H8O6
Mol. Weight: 176.13


Properties

Form: Powder
Appearance: White Crystalline
Application: Antioxidant; Vitamin
Solubility: Water
Typical Working Concentration: Varies with application, should be determined by end user.
Storage Temp: Room Temperature
Other Notes: Plant Tissue Culture Tested. Heat-sensitive, do not autoclave.

Application Notes
Commonly used to overcome browning of tissues in plant tissue culture.

References
Banerjee N & Langhe E (1985) A tissue culture technique for rapid clonal propagation and storage under minimal growth conditions of Musa (banana and plantain). Plant Cell Reports, 4(6), 351-354.

Joy Iv R.W., Patel K.R. & Thorpe T.A. (1988) Ascorbic acid enhancement of organogenesis in tobacco. Plant Cell Tissue Organ Cult. 13, 219-228.

Ko, W. H., Su, C. C., Chen, C. L., & Chao, C. P. (2009). Control of lethal browning of tissue culture plantlets of Cavendish banana cv. Formosana with ascorbic acid. Plant Cell, Tissue and Organ Culture, 96(2), 137-141.

Stasolla, C., & Yeung, E. C. (1999). Ascorbic acid improves conversion of white spruce somatic embryos. In Vitro Cellular & Developmental Biology-Plant, 35(4), 316-319. Merck 13, 837.

Formula (mg/L)
Boric Acid 143
Calcium Chloride Anhydrous 1359
Citric Acid, Anhydrous 300
Cobalt Nitrate·6H2O 2.45
Cupric Sulfate∙5H2O 3.95
Ferric Ammonium Citrate 300
Magnesium Sulfate∙7H2O 3750
Manganese Chloride∙4H2O 90.5
Na2∙Mg∙EDTA 50
Potassium Phosphate dibasic 2000
Sodium Carbonate Anhydrous 1000
Sodium Molybdate∙2H2O 19.5
Sodium Nitrate 75000
Zinc Sulfate*7H2O 0.222
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