Enquiry E-CATALOGUE

Acetosyringone

  • Product ID: A104

Introduction:

Acetosyringone is a naturally occurring compound secreted from wounded dicot plant tissues and is a chemotractant for Agrobacterium sp.. It is generally used at 5uM to 200uM to enhance transformation in monocots, 3',5'-Dimethyoxy-4'-hydroxyacetophenone Acetosyringone should NOT be autoclaved.

A104 Acetosyringone

Synonyms: 4-Hydroxy-3’,5’-dimethoxyacetophenone
CAS: 2478-38-8
Formula: C10H12O4
Mol. Weight: 196.20


Properties

Form: Powder
Appearance: Off-white to Tan
Application: Molecular Biology
Solubility: DMSO
Typical Working Concentration: 50 µM to 200 µM
Storage Temp: Room Temperature
Storage Temp of Stock Solution: -20 to 0ºC
Other Notes: Plant Tissue Culture Tested. Heat sensitive, do not autoclave.

Application Notes
Acetosyringone is a naturally occurring compound secreted from wounded plant tissues. It is used to increase the rate of transformation when used at 50 µM to 200 µM. (Sheikholeslam & Weeks, 1987).

References
João KHL and TA Brown (1993) Enhanced transformation of tomato co-cultivated with Agrobacterium tumefaciens C58C1Rifr::pGSFR1161 in the presence of acetosyringone. Plant Cell Reports, 12:7-8, pp. 422-425. Mathews H, Bharathan N, Litz RE, Narayanan KR, Rao PS and Bhatia CR (1990) The promotion of Agrobacterium mediated transformation in Atropa belladonna L. by acetosyringone. Journal of Plant Physiology, 136 (4). pp. 404-409. ISSN 0176-1617. Sheikholeslam, SN & DP Weeks. 1987. Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens. Plant Molecular Biology 8:291-298.

Formula (mg/L)
Boric Acid 143
Calcium Chloride Anhydrous 1359
Citric Acid, Anhydrous 300
Cobalt Nitrate·6H2O 2.45
Cupric Sulfate∙5H2O 3.95
Ferric Ammonium Citrate 300
Magnesium Sulfate∙7H2O 3750
Manganese Chloride∙4H2O 90.5
Na2∙Mg∙EDTA 50
Potassium Phosphate dibasic 2000
Sodium Carbonate Anhydrous 1000
Sodium Molybdate∙2H2O 19.5
Sodium Nitrate 75000
Zinc Sulfate*7H2O 0.222
Life Technologies (India) Pvt Ltd. 

306, Agarwal City Mall, opposite M2K Pitampura, Delhi-110034 (India)
Tel # +91-11-4220-8000; 4220-8111; 4220-8222 Fax# +91-11-4220-8444,
Mobile# +91-98105-21400, Toll Free# 1800-120-2434
Email# customerservice@lifetechindia.com